CRISPR-CasN gene Editing
CRISPR-CasN is our next-generation gene editing service platform, featuring higher knock-in efficiency, lower off-target effects, and reduced immunogenicity compared to SpCas9.
We offer a comprehensive one-stop service covering:
1. Knock-out: Supports both single-gene and multi-gene knock-out, delivering sequencing-validated biallelic knock-out cell lines or cell pools within just 5–12 weeks.
2. Knock-in: Includes point mutations, seamless point mutations, endogenous locus tagging, reporter gene insertion, cell models for genetic variations (SNV, SV, InDel), and targeted site-specific integration of genes of interest.
3. Design Services: Combining advanced algorithms with the latest genomic databases, we provide sgRNA+ repairing template design and pegRNA design (leveraging Prime Editing technology) to overcome the sequence limitations of traditional CRISPR. We also offer one-stop Cas12 diagnostic assay design, covering enzyme selection, reporter probe design, and functional validation.
4. CRISPR Reagents: We also supply CasN Protein, CasN Plasmid, and high-purity sgRNA to support all your R&D and production needs.
Through our strategic partnership with UK-based Prime.io, we bring cutting-edge automated gene editing technology directly to you. This collaboration is further empowered by Sir Tony Kouzarides—a pioneering figure in the Cambridge life sciences ecosystem and Strategic Advisor to Prime.io—ensuring unparalleled precision in our cell line engineering.
Whether you are working with conventional cell lines or hard-to-transfect cells such as primary cells and stem cells, we deliver precise and reproducible gene editing solutions tailored to your needs.
Choose CRISPR-CasN – for more efficient, safer, and hassle-free gene editing research.
Our Dedicated Scientific Leadership
Rex Li Ph.D.
Chief Scientist
Graduated from King's College, University of Cambridge, UK.
Extensive experience in the field of gene editing. Responsible for the company's gene editing R&D strategy, continuously driving the development and optimization of novel CRISPR systems and delivery technologies.
E-mail：Rex@upperbiotech.com
Loren Yang
Scientist
Extensive experience in molecular diagnostics and gene editing.
Proficient in CRISPR-based detection technologies and advanced molecular assay development.
E-mail: LorenYang@upperbiotech.com
Jessie Xu Ph.D.
Manager, International Business
Graduated from the University of Cambridge, UK.
Excellent cross-cultural communication skills, quick mastery of complex international trade processes.
E-mail：jessie@upperbiotech.com
Product Information
CRISPR-CasN Knock-in/Knock-out Service
The CRISPR-CasN delivery system features higher knock-in efficiency, lower off-target effects, and reduced immunogenicity compared to SpCas9.
Service Type
Service Details
Cell Line Options
Deliverables
Turnaround Time
Gene Knock-out (KO)
Single or multi-gene target biallelic KO cell pool or single clone.
Standard cell lines, hard-to-transfect primary cells, and stem cells.
Validated cell line/pool & complete QC sequencing report.
5 - 8 Weeks
Gene Knock-in (KI)
Point mutation (SNV), seamless tag insertion, or targeted gene integration.
Broad compatibility with immortalized and specialized cell models.
Validated KI cell line & comprehensive validation summary.
8 - 12 Weeks
sgRNA+ repairing template design Service
Our service system comprehensively covers SpCas9 knockout/knock-in experiments and CRISPR interference/activation applications, deeply integrating next-generation machine learning tools with the extensive expertise of our senior scientific team to ensure editing efficiency and specificity from the very start, thereby maximizing your experimental success rate. Whether your project requires precise single-target editing or high-throughput functional screening, we can tailor either single sgRNA or pooled sgRNA library solutions to fully meet your research needs.
pegRNA Design Service
Leveraging leading Prime Editing technology, our service employs intelligent algorithms to efficiently design pegRNAs, enabling easy substitution, insertion, or deletion of any base, breaking through the sequence limitations of traditional CRISPR.
Cas12 Diagnostic Assay Experimental Design Service
We offer you a one-stop solution covering Cas12 enzyme selection, custom ssDNA reporter probe design, high-specificity sgRNA design, functional assay validation, and standardized experimental workflow development—making your diagnostic development more precise and efficient.
CRISPR Reagents
CasN Protein
Combining high cleavage activity with low off-target risk, providing a stable and reliable core enzyme for your research and therapeutic development.
CasN Plasmid
The CasN plasmid is equipped with multiple elements (promoters/tags/selection markers), easily adaptable to mammalian, plant, or microbial systems, creating your exclusive gene editing tool.
sgRNA（plasmid）
High-quality sgRNA delivered directly to your lab bench, saving time and effort while accelerating your gene editing and screening processes.
Partner
Prime.io (United Kingdom)
Our strategic partner based in London, providing next-generation cell line engineering services.
Prime.io leverages AI-designed CRISPR technology and automation to deliver high-speed, high-quality gene knock-in (KI) and knock-out (KO) services. Their platform significantly accelerates drug discovery pipelines for our clients.
The Prime.io scientific advisory board is led by Sir Tony Kouzarides (Co-founder of Abcam, Milner Therapeutics Institute; Venture Partner at Fifty Years), ensuring world-class scientific rigor behind our joint offerings.
Inquiry Form
Please fill out the form below for gene editing or clinical service inquiries. Our specialist will contact you shortly.