First Fractions :
EV + larger
particles
Last Fractions:
smaller particles
Principle
>90%
EV Recovery
High recovery to maximize EV yield
>99%
Protein Removal
Efficient removal of soluble
protein from human plasma
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98% LDL removal
99.99% HDL removal
Liproprotein Removal
Dual EV Quantification by NTA and BCA
EV particles quantified by NTA and EV-associated protein measured via BCA co-elute, with both peaks appearing in early SEC fractions.
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100
200
300
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95% of Isolated EVs Are Within the 200 nm Range
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EV Purity from Fluorescent Standard
High EV Purity from Human Plasma
SEC fractionation gently removes ~99% of soluble protein from human plasma, enriching intact EVs.
This delivers high particle-to-protein ratio, a key metric demonstrating purified EV sample purity.
Efficient Lipoprotein Separation
Lipoproteins are largely separated from the EV-rich fractions, with HDL/ApoA-I and LDL/ApoB concentrated in later SEC fractions.
This enables efficient collection of EV-rich fractions with reduced lipoprotein contamination.
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Lipophilic DiI inserts into EV lipid membranes and co-elutes entirely within EV-rich SEC fractions. This verifies all DiI signal is EV-associated, confirming high purity of the labelled EV population.
DiI Dye Labelling
EV Dye Labelling
Column size
Standard: up to 1.5mL loading volume
Maxi: up to 5mL loading volume
Format
Single use or reusable up to 5 times
Bed Volume
Standard: 10mL
Maxi: 30mL
Manual or integrated with the ExoFaster 500 for automated EV isolation
Maximum 9 Standard columns per run
Maximum 7 Maxi columns per run
Compatibility
Relative Yield Purity & Yield Over Reuse
Consistent EV yield and purity are preserved across repeated runs relative to first-use performance.
Supports reproducible EV separations thanks to stable resin chemistry.
5
reuses per column
<5%
CV in EV Field
Engineered for a stable and consistent dripping speed throughout the SEC separation process.
Reliable flow performance supports reproducible EV isolation and consistent downstream results.
Consistent Flow Rate
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Composite Series
Complex or protein-rich samples
Ultra-High Purity
-
High purity EV preparation
-
Better suited for protein-rich samples
-
Suitable for proteomics / LC-MS
-
Focus: Maximize Purity
Highest purity
P Series
Balanced Recovery & Purity
-
Balanced recovery and purity
-
Suitable for routine EV isolation
-
Flexible for protein and RNA analysis
-
Focus: Balanced Performance
Routine EV samples
Y Series
Low - EV or precious samples
High Recovery
-
High EV recovery
-
Suitable when sample amount is limited
-
Ideal for general EV characterization
-
Focus: Maximize Recovery
Highest yield
Low EV or precious samples
Composite series
Y series
Match Your
Sample to the Right
EV Series
Complex or protein-rich samples
Ultra-high purity
Balanced recovery and purity
High recovery
P Series
Sample
Compatibility
Recovery
Purity
Human Serum albumin depletion
HDL (ApoA-I) depletion
LDL (ApoB) depletion
Resin
Y Series
P Series
> 99.9%
> 99%
> 40%
Composite Series
30nm SEC
> 99.9%
> 99%
> 80%
50nm SEC
Composite: SEC + ligand-activated core-shell resin
> 99.9%
> 99%
> 99%
Beyond Size-Based Separation
EVs and ApoB-containing lipoproteins such as LDL and VLDL occupy overlapping size ranges, making conventional SEC alone insufficient for clean separation.
Our Composite Series adds hydrophobic binding and ion-exchange interactions to SEC, creating multiple layers of separation to more effectively remove lipoprotein contaminants while preserving EVs.
Milk
Column
Particle/mL
Standard-Y
3.45E+11
Standard-P
Protein mg/mL
0.103
Particle/ug Protein
3.35E+09
1.90E+11
0.18
1.06E+10
Maxi-Y
3.28E+11
0.089
3.69E+09
1.74E+11
Maxi-P
0.014
1.24E+10
Conditioned Medium 1
Standard-C
Column
Particle/mL
Protein mg/mL
Particle/ug Protein
5.37E+10
0.140
3.84E+08
6.45E+09
Standard-Y
1.61E+09
5.49E+11
0.004
Maxi-Y
0.153
3.59E+08
5.46E+09
Maxi-P
0.002
2.73E+09
Conditioned Medium 2
Standard-Y
Column
Particle/mL
Protein mg/mL
Particle/ug Protein
1.05E+11
0.354
2.97E+08
Standard-P
4.18E+10
0.103
4.06E+08
Maxi-Y
1.25E+11
0.396
3.16E+08
Maxi-P
4.35E+10
0.115
3.78E+08
Chlorella Conditioned Medium
Standard-Y
Column
Particle/mL
Protein mg/mL
Particle/ug Protein
3.13E+11
0.257
1.22E+09
Standard-P
3.39E+11
0.271
1.25E+09
Maxi-Y
3.25E+11
0.264
1.23E+09
3.54E+11
Maxi-P
0.283
1.25E+09
Human Plasma
Standard-Y
Column
Particle/mL
Protein mg/mL
Particle/ug Protein
5.96E+11
0.846
7.04E+08
Standard-P
1.34E+11
0.139
9.04E+08
5.71E+11
Maxi-Y
0.727
7.85E+08
1.45E+11
Maxi-P
0.153
9.48E+08
Inner Packaging
Outer Packaging
